UJI AKTIVITAS NEUROPROTEKTIF ISOLAT ANDROGRAFOLID SECARA IN VITRO MELALUI PENGHAMBATAN ENZIM ASETILKOLINESTERASE (AChE) DENGAN MENGGUNAKAN METODE ELISA

DILLA NUR YULIANI, - (2025) UJI AKTIVITAS NEUROPROTEKTIF ISOLAT ANDROGRAFOLID SECARA IN VITRO MELALUI PENGHAMBATAN ENZIM ASETILKOLINESTERASE (AChE) DENGAN MENGGUNAKAN METODE ELISA. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Penyakit neurodegeneratif, termasuk Alzheimer, ditandai dengan penurunan progresif fungsi kognitif akibat kerusakan sel saraf. Salah satu mekanisme terapi Alzheimer adalah penghambatan enzim asetilkolinesterase (AChE) yang berperan dalam degradasi asetilkolin. Penelitian ini bertujuan untuk mengevaluasi aktivitas neuroprotektif isolat andrografolid dari tanaman Andrographis paniculata (sambiloto) melalui penghambatan enzim AChE secara in vitro menggunakan metode Enzyme-Linked Immunosorbent Assay (ELISA). Isolat andrografolid diuji pada konsentrasi 0,1; 1; 10; dan 100 ppm, dengan kontrol positif donepezil (0,08 µM). Hasil uji menunjukkan bahwa isolat andrografolid mampu menghambat aktivitas AChE dengan persentase inhibisi yang tinggi pada seluruh konsentrasi uji. Nilai absorbansi menurun signifikan dibandingkan dengan kontrol negatif, yang menunjukkan efektivitas penghambatan enzim oleh isolat. Dengan demikian, isolat andrografolid berpotensi sebagai kandidat agen neuroprotektif alami untuk terapi penyakit Alzheimer. ----- Neurodegenerative diseases, including Alzheimer’s disease, are characterized by a progressive decline in cognitive function caused by neuronal damage. One therapeutic mechanism for Alzheimer’s disease is the inhibition of acetylcholinesterase (AChE), an enzyme responsible for the degradation of acetylcholine. This study aimed to evaluate the neuroprotective activity of andrographolide isolated from Andrographis paniculata (sambiloto) through AChE inhibition in vitro using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The andrographolide isolate was tested at concentrations of 0.1, 1, 10, and 100 ppm, with donepezil (0.08 µM) as the positive control. The results showed that the andrographolide isolate was able to inhibit AChE activity with a high percentage of inhibition at all tested concentrations. The absorbance values significantly decreased compared to the negative control, indicating effective enzyme inhibition by the isolate. Therefore, the andrographolide isolate has potential as a natural neuroprotective candidate for Alzheimer’s disease therapy.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Alzheimer, Asetilkolinesterase, Andrografolid, Metode ELISA. ------- Alzheimer’s disease, Acetylcholinesterase, Andrographolide, ELISA method
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	19 Nov 2025 08:06
Last Modified:	19 Nov 2025 08:06
URI:	http://repository.stfi.ac.id/id/eprint/3508

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