AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI HERBA RUMPUT MUTIARA (Oldenlandia corymbosa L.) MENGGUNAKAN METODE DPPH

DELYVIA APRILLYANTI, - (2025) AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI HERBA RUMPUT MUTIARA (Oldenlandia corymbosa L.) MENGGUNAKAN METODE DPPH. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Herba rumput mutiara mengandung senyawa aktif seperti alkaloid, flavonoid, saponin, fenolik dan triterpenoid. Tujuan penelitian ini adalah untuk mengetahui aktivitas antioksidan serta menentukan nilai IC₅₀ dari ekstrak dan fraksi herba rumput mutiara (Oldenlandia corymbosa L.) dalam meredam radikal bebas. Uji aktivitas antioksidan dilakukan menggunakan metode DPPH (2,2-difenil-1- pikrilhidrazil). Pengujian dilakukan terhadap ekstrak etanol, fraksi n-heksan, fraksi etil-asetat, dan fraksi air herba rumput mutiara. Nilai IC₅₀ ditentukan melalui analisis regresi dari serapan pada panjang gelombang dengan menggunakan instrumen spektrofotometer UV-Vis. Hasil penelitian menunjukkan nilai IC₅₀ dari ekstrak, fraksi n-heksan, fraksi etil-asetat adalah 22,7896 ppm, 23,3290 ppm, 13,9477 ppm dan 19,0273 ppm secara berturut-turut. Ekstrak dan fraksi herba rumput mutiara memiliki aktivitas antioksidan dengan kategori sangat kuat. ------ Pearl grass herb contains active compounds such as alkaloids, flavonoids, saponins, phenolics and triterpenoids. The purpose of this study was to determine the antioxidant activity and determine the IC₅₀ value of the extract and fraction of pearl grass herb (Oldenlandia corymbosa L.) in reducing free radicals. The antioxidant activity test was carried out using the DPPH (2,2-diphenyl-1- picrylhydrazyl) method. Tests were carried out on ethanol extracts, n-hexane fractions, ethyl-acetate fractions and water fractions of pearl grass herb. The IC₅₀ value was determined through regression analysis of absorption at wavelengths using a UV-Vis spectrophotometer instrument. The results showed that the IC₅₀ value of the extract, n-hexane fraction, ethyl-acetate fraction was 22.7896 ppm, 23.3290 ppm, 13.9477 ppm and 19.0273 ppm respectively. Pearl grass herb extracts and fractions have antioxidant activity in the very strong category.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Herba rumput mutiara, antioksidan, DPPH. ----- Oldenlandiae corymbosa, antioxidant, DPPH.
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Program Studi S1 Farmasi
Depositing User: pustakawan - -
Date Deposited: 19 Nov 2025 02:20
Last Modified: 19 Nov 2025 02:20
URI: http://repository.stfi.ac.id/id/eprint/3483

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