In Silico Study of Single Chain Fragment Variable Antibody and Indonesian Serotype-2 NS1 Dengue Virus Antigen

DEWI ASTRIANY, - and YENI INDAH PUSPITA SARI, - and UMI BAROROH, - and MUHAMMAD YUSUF, - and IMAN PERMANA MAKSUM, - and DESY NATALIA, - and TOTO SUBROTO, - (2020) In Silico Study of Single Chain Fragment Variable Antibody and Indonesian Serotype-2 NS1 Dengue Virus Antigen. In: The proceedings of the 2nd Bakti Tunas Husada-Health Science International Conference (BTH-HSIC 2019).

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Abstract

Objectives: The aim of this study was to determine the interaction between Indonesian NS1 DENV-2 with 6D4B10 and 8A6F2 single chain fragment variable antibodies. Method: In this study, bioinformatics approach was performed on the six structures of Indonesian NS1 antigenes of DENV-2 to obtain the best model using BLAST, Pyre2, Modeller 9.20 and the FireDock server for molecular docking. Results and Discussion: We obtained the binding energy of 6D4B10 antibody to NS1 D2/ID/SMG-SE001 was -55.04 kcal/mol and 8A6F2 antibody to D2/ID/JKT-J002 was -49.42 kcal/mol. The interactions of antigen-antibody complex were hydrogen bonds, electrostatic interactions, hydrophobic interactions and several other interactions. Conclusion: The results showed that 6D4B10 and 8A6F2 antibodies can be provide for designing molecular antibody fragments as one of dengue diagnostic kits agent in Indonesia.

Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	dengue virus, antigen, antibody, molecular docking
Subjects:	Q Science > QR Microbiology R Medicine > R Medicine (General)
Divisions:	Lembaga Penelitian dan Pengabdian Masyarakat > Publikasi Penelitian
Depositing User:	pustakawan - -
Date Deposited:	04 Jul 2024 08:19
Last Modified:	16 Aug 2024 01:50
URI:	http://repository.stfi.ac.id/id/eprint/344

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