EFEKTIVITAS KONDISI SUHU ANNEALING PCR GEN SURFACTIN SYNTHASE DARI BAKTERI Bacillus cereus

TAUFIK HIDAYAT, - (2023) EFEKTIVITAS KONDISI SUHU ANNEALING PCR GEN SURFACTIN SYNTHASE DARI BAKTERI Bacillus cereus. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Biosurfaktan merupakan komponen mikroba yang terdiri dari molekul hidrofilik dan hidrofobik yang mampu membuat ikatan dengan molekul hidrokarbon yang tidak larut air, serta membuat tegangan permukaan menjadi turun. Bacillus cereus berpotensi sebagai penghasil biosurfaktan golongan lipopeptida. Penelitian ini bertujuan mendapatkan keseluruhan genom Bacillus cereus dan mendapat kondisi optimum suhu penempelan primer yang digunakan untuk memunculkan gen surfactin synhase dengan metode Polymerase Chain Reaction (PCR). Penelitian ini dimulai dengan meremajakan bakteri Bacillus cereus, isolasi genom menggunakan PrestoTM Mini gDNA Bacteria kit, dan perbanyakan gen target melalui PCR. Analisis genom dan fragmen DNA dari gen dilakukan dengan menggunakan elektroforesis gel agarosa. Didapatkan analisis hasil visualisasi elektroforesis genom dengan ukuran lebih dari 10 kb. Deteksi gen pengkode pengkode surfactin synthase pada gel agarose 1% menunjukkan hasil pasangan primer srfA4f,srfA4R T annealing 57oC terdapat pita DNA 2 kb dan srfB5F, srfB5R pada T annealing 57oC terdapat pita berukuran 1,5 kb, dan srfA3R di T annealing 61,6oC menunjukkan terdapat pita DNA berukuran 2 kb. --- Biosurfactants are microbial components consisting of hydrophilic and hydrophobic molecules that can form bonds with hydrocarbon molecules that are insoluble in water, as well as reduce the surface tension. Bacillus cereus holds potential as a producer of lipopeptide-type biosurfactants. This study aims to obtain the complete genome of Bacillus cereus and determine the optimal primer annealing temperature for amplifying the surfactin synthase gene using the Polymerase Chain Reaction (PCR) method. The research begins with the cultivation of Bacillus cereus bacteria, genome isolation using the PrestoTM Mini Bacteria gDNA Kit, and amplification of the target gene through PCR. Genomic analysis and DNA fragment analysis of the gene are performed using agarose gel electrophoresis. The results reveal the visualization analysis of the genomic electrophoresis, showing fragments larger than 10 kb. Detection of the surfactin synthase-encoding gene on a 1% agarose gel demonstrates that the srfA4f-srfA4R primer pair at 57°C T annealing temperature yields a 2 kb DNA band, while srfB5F-srfB5R at 57°C T annealing temperature yields a 1.5 kb band, and srfA3R at a 61.6°C annealing temperature shows a 2 kb DNA band.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Ekstraksi DNA, Polymerase Chain Reaction, Elektroforesis, T annealing DNA Extraction, Polymerase Chain Reaction, Electrophoresis, T annealing,
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	21 Jun 2024 06:49
Last Modified:	21 Jun 2024 06:49
URI:	http://repository.stfi.ac.id/id/eprint/281

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