SALSA HASNA MUTIA, - (2025) UJI AKTIVITAS NEUROPROTEKTIF ISOLAT ANDROGRAFOLID DARI TANAMAN SAMBILOTO (Andrographis paniculata Nees.) SEBAGAI INHIBITOR ENZIM ASETILKOLINESTERASE (AChE) SECARA IN VITRO MENGGUNAKAN REAKSI ELLMAN. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Penyakit Alzheimer merupakan gangguan neurodegeneratif progresif yang ditandai oleh penurunan fungsi kognitif akibat aktivitas enzim asetilkolinesterase (AChE) yang berlebihan. Penghambatan AChE menjadi pendekatan terapeutik penting untuk meningkatkan kadar asetilkolin di sinapsis. Penelitian ini bertujuan untuk mengevaluasi potensi isolat andrografolid dari tanaman sambiloto (Andrographis paniculata Nees.) sebagai inhibitor enzim AChE secara in vitro menggunakan reaksi Ellman. Aktivitas inhibisi diukur melalui persentase inhibisi (% inhibisi) pada empat konsentrasi bertingkat (0,1; 1; 10; dan 100 ppm), dengan kontrol positif Donepezil HCl 0,08 µM. Hasil menunjukkan bahwa isolat andrografolid menghasilkan % inhibisi tertinggi sebesar 80,90% pada konsentrasi 100 ppm, dan mendekati efektivitas kontrol positif pada konsentrasi 10 ppm (49,76%). Data % inhibisi digunakan untuk menghitung nilai IC50 melalui regresi linier, dengan persamaan y = 0,4437x + 37,314 dan koefisien determinasi R² = 0,933. Nilai IC50 yang diperoleh sebesar 28,591 ppm menunjukkan potensi penghambatan yang sangat kuat terhadap enzim AChE. Hubungan antara % inhibisi dan IC50 memperkuat validitas data sebagai parameter kuantitatif dalam penilaian efektivitas senyawa uji. Temuan ini memberikan dasar ilmiah bahwa isolat andrografolid memiliki potensi sebagai kandidat inhibitor AChE dalam pengembangan terapi Alzheimer berbasis bahan alam. ----- Alzheimer’s disease is a progressive neurodegenerative disorder characterized by cognitive decline, primarily driven by excessive activity of the enzyme acetylcholinesterase (AChE). Inhibiting AChE is a key therapeutic strategy to increase acetylcholine levels at synapses. This study aimed to evaluate the inhibitory potential of andrographolide isolate from Andrographis paniculata Nees. against AChE in vitro using the Ellman reaction. Enzyme inhibition was assessed through percentage inhibition (% inhibition) at four graded concentrations (0.1; 1; 10; and 100 ppm), with Donepezil HCl 0.08 µM as the positive control. The isolate showed the highest % inhibition of 80.90% at 100 ppm and a comparable effect to the control at 10 ppm (49.76%). The % inhibition data were used to calculate the IC₅₀ value via linear regression, yielding the equation y = 0.4437x + 37.314 with a determination coefficient R² = 0.933. The resulting IC₅₀ value of 28.591 ppm indicates very strong inhibitory potency. The correlation between % inhibition and IC₅₀ reinforces the validity of both parameters as quantitative indicators of enzyme inhibition. These findings support the potential of andrographolide isolate as a natural AChE inhibitor and provide a scientific basis for its further development in Alzheimer’s therapy.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Alzheimer, Andrografolid, Asetilkolinesterase, IC₅₀, Ellman. ------ Alzheimer’s, Andrographolide, Acetylcholinesterase, IC₅₀, Ellman’s. |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 09 Sep 2025 03:05 |
| Last Modified: | 09 Sep 2025 03:05 |
| URI: | http://repository.stfi.ac.id/id/eprint/2776 |
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