NITA YUNI KARTIKA, - (2025) AKTIVITAS NEUROPROTEKTIF ISOLAT ANDROGRAFOLID TERHADAP PROLIFERASI DAN DIFERENSIASI SEL-SEL OTAK BESAR ANAK TIKUS PADA MEDIUM DASAR mDMEM (Dulbecco’s Modified Eagle’s Medium). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A233017_Title.pdf Download (3MB) |
|
Text
S_PSSF_A233017_Chapter1.pdf Restricted to Repository staff only Download (815kB) |
|
Text
S_PSSF_A233017_Chapter2.pdf Restricted to Repository staff only Download (3MB) |
|
Text
S_PSSF_A233017_Chapter3.pdf Restricted to Repository staff only Download (1MB) |
|
Text
S_PSSF_A233017_Chapter4.pdf Restricted to Repository staff only Download (2MB) |
|
Text
S_PSSF_A233017_Chapter5.pdf Restricted to Repository staff only Download (240kB) |
|
Text
S_PSSF_A233017_Appendix.pdf Restricted to Repository staff only Download (12MB) |
Abstract
Penyakit neurodegeneratif merupakan penyakit yang dialami oleh seseorang ditandai dengan hilangnya memori dan kemampuan kognitif. Neurodegeneratif dapat terjadi karena adanya neuroinflamasi yang disebabkan salah satunya akibat adanya gangguan pada proliferasi dan diferensiasi pada sel otak. Andrografolid merupakan senyawa marker atau komponen utama yang berasal dari tanaman sambiloto (Andrographis paniculata) dan termasuk golongan terpenoid. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh pemberian isolat andrografolid terhadap proliferasi dan diferensiasi sel-sel otak besar. Pada penelitian ini otak anak tikus dirusak dengan cara dibuat suspensi kemudian dibagi menjadi 5 kelompok yang terdiri dari kelompok normal (tanpa diberikan perlakuan), kelompok kontrol positif (dengan penambahan Taurin 100 ppm), serta 3 kelompok uji yang diberikan perlakuan isolat andrografolid dengan dosis masing-masing 100 ppm, 200 ppm, dan 400 ppm. Dalam penelitian ini hasil yang diperoleh menunjukkan bahwa isolat andrografolid mampu memberikan aktivitas neuroprotektif terhadap proliferasi dan diferensiasi sel-sel otak besar. Dosis isolat andrografolid yang aktivitasnya paling optimal yaitu pada dosis 200 ppm. Andrografolid bekerja dengan meningkatkan kemampuan aktivitas neuron melalui jalur pensinyalan yang dimediasi NFkB sehingga dapat mengurangi kadar mediator inflamasi untuk melindungi neuron dari kerusakan yang dihasilkan oleh peradangan. ------- Neurodegenerative disease is a disease experienced by a person characterized by memory loss and cognitive abilities. Neurodegenerative can occur due to neuroinflammation caused by, among other things, disorders in the proliferation and differentiation of brain cells. Andrographolide is a marker compound or main component derived from the bitter plant (Andrographis paniculata) and belongs to the terpenoid group. The purpose of this study was to determine the effect of administering andrographolide isolate on the proliferation and differentiation of cerebrum cells. In this study, the brains of pups were damaged by making a suspension and then divided into 5 groups consisting of a normal group (without treatment), a positive control group (with the addition of 100 ppm Taurine), and 3 test groups treated with andrographolide isolate at doses of 100 ppm, 200 ppm, and 400 ppm, respectively. In this study, the results obtained showed that andrographolide isolate was able to provide neuroprotective activity against the proliferation and differentiation of cerebrum cells. The dose of andrographolide isolate with the most optimal activity was at a dose of 200 ppm. Andrographolide works by increasing the activity of neurons through the NFkB-mediated signaling pathway, thereby reducing the levels of inflammatory mediators to protect neurons from damage caused by inflammation.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Andrografolid, Medium mDMEM, Neurodegeneratif, Proliferasi dan Diferensiasi. ------ Andrographolide, mDMEM Medium, Neurodegenerative, Proliferation and Differentiation |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 04 Sep 2025 02:42 |
| Last Modified: | 04 Sep 2025 02:42 |
| URI: | http://repository.stfi.ac.id/id/eprint/2759 |
Actions (login required)
 |
View Item |
