NABILA SITI AINURHIKMAT, - (2025) ISOLASI DAN IDENTIFIKASI BAKTERI ASOSIASI SPONS LAUT Agelas citrina YANG BERPOTENSI SEBAGAI ANTIBAKTERI DARI KEPULAUAN SERIBU JAKARTA. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Permasalahan resistensi antimikroba (AMR) yang semakin meningkat menjadi tantangan serius dalam penanganan infeksi bakteri patogen, sehingga diperlukan sumber antibakteri baru yang efektif dan aman. Spons laut Agelas citrina dari perairan Kepulauan Seribu diduga memiliki bakteri asosiasi yang mampu menghasilkan senyawa antibakteri potensial. Penelitian ini bertujuan mengisolasi dan mengidentifikasi bakteri asosiasi spons Agelas citrina yang berpotensi sebagai antibakteri serta menguji aktivitas antibakteri terhadap bakteri patogen Escherichia coli dan Staphylococcus aureus. Metode yang digunakan meliputi isolasi dan purifikasi bakteri dari spons menggunakan media Zobell Marine Agar, identifikasi morfologi dan pewarnaan Gram, uji aktivitas antibakteri menggunakan metode difusi cakram, serta identifikasi molekuler melalui sekuensing Gen 16S rRNA. Hasil penelitian menunjukkan terdapat 11 isolat bakteri, mayoritas berbentuk kokobasil gram positif dan satu isolat berbentuk Vibrio gram negatif. Isolat kode SA 104 teridentifikasi secara molekuler sebagai Vibrio alginolyticus dan menunjukkan aktivitas antibakteri kuat terhadap E. coli (zona hambat 17 mm) dan sedang terhadap S. aureus (zona hambat 6,7 mm). Bakteri asosiasi pada spons laut Agelas citrina memiliki potensi sebagai sumber antibakteri baru untuk pengembangan obat antimikroba. ------ The increasing problem of antimicrobial resistance (AMR) poses a serious challenge in treating bacterial infections, thus necessitating the discovery of new, effective, and safe antibacterial agents. The marine sponge Agelas citrina from the Thousand Islands, Jakarta, is suspected to harbor associated bacteria capable of producing potential antibacterial compounds. This study aims to isolate and identify bacteria associated with Agelas citrina that have antibacterial potential and to test their antibacterial activity against the pathogenic bacteria Escherichia coli and Staphylococcus aureus. The methods used included isolation and purification of bacteria from the sponge using Zobell Marine Agar media, morphological and Gram staining identification, antibacterial activity assay using the disc diffusion method, and molecular identification through 16S rRNA gene sequencing. The results revealed 11 bacterial isolates, the majority being grampositive coccobacilli and one isolate identified as gram-negative Vibrio. The isolate coded SA 104 was molecularly identified as Vibrio alginolyticus and exhibited strong antibacterial activity against E. coli (inhibition zone of 17 mm) and moderate activity against S. aureus (inhibition zone of 6.7 mm). In conclusion, bacteria associated with the marine sponge Agelas citrina have potential as new sources of antibacterial agents for antimicrobial drug development.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Agelas citrina, bakteri asosiasi, antibakteri, Vibrio alginolyticus, Gen 16S rRNA, resistensi antimikroba, spons laut, isolasi bakteri. ----- Agelas citrina, sponge-associated bacteria, antibacterial, Vibrio alginolyticus, 16S rRNA gene, antimicrobial resistance, marine sponge, bacterial isolation. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 04 Sep 2025 01:49 |
| Last Modified: | 04 Sep 2025 01:49 |
| URI: | http://repository.stfi.ac.id/id/eprint/2754 |
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