EVALUASI POTENSI TERATOGENIK ISOLAT ANDROGRAFOLID MELALUI LIMIT TEST PADA MENCIT BETINA SWISS WEBSTER DENGAN METODE OECD 414

MELDHY SESILYA, - (2025) EVALUASI POTENSI TERATOGENIK ISOLAT ANDROGRAFOLID MELALUI LIMIT TEST PADA MENCIT BETINA SWISS WEBSTER DENGAN METODE OECD 414. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Andrographis paniculata (sambiloto) dikenal memiliki berbagai efek farmakologis, namun keamanan penggunaannya pada kehamilan belum banyak diteliti. Kandungan aktif utamanya, andrografolid, diduga memiliki potensi teratogenik yang dapat mengganggu perkembangan janin. Penelitian ini bertujuan mengevaluasi efek teratogenik isolat andrografolid terhadap perkembangan fetus mencit betina Swiss Webster. Uji dilakukan menggunakan metode OECD 414 dengan desain limit test pada dosis 2000 mg/kgbb yang diberikan secara oral selama periode organogenesis (hari ke-6 hingga ke-15 kebuntingan). Parameter yang diamati meliputi bobot dan panjang fetus, kelainan morfologi (tubuh kerdil, bongkok, hemoragi, dan kelopak mata terbuka), serta pemeriksaan histopatologi hati induk mencit. Hasil penelitian menunjukkan kelompok perlakuan mengalami penurunan signifikan bobot dan panjang fetus dibanding kontrol, serta peningkatan kelainan morfologi tersebut. Pemeriksaan histopatologi hati induk mencit menunjukkan kerusakan ringan hingga sedang yang bersifat reversibel. Disimpulkan bahwa isolat andrografolid pada dosis tinggi memiliki potensi menyebabkan gangguan pertumbuhan dan kelainan morfologi janin, sehingga penggunaannya pada kehamilan perlu diwaspadai. ----- Andrographis paniculata (sambiloto) is known for its various pharmacological effects; however, its safety during pregnancy has not been extensively studied. Its main active compound, andrographolide, is suspected to possess teratogenic potential that may interfere with fetal development. This study aimed to evaluate the teratogenic effects of andrographolide isolate on the development of female Swiss Webster mouse fetuses. The test was conducted using the OECD 414 method with a limit test design at a dose of 2000 mg/kg bw administered orally during the organogenesis period (day 6 to day 15 of gestation). Observed parameters included fetal body weight and length, morphological abnormalities (stunted body, hunchback posture, hemorrhage, and open eyelids), as well as histopathological examination of maternal liver tissue. The results showed that the treatment group experienced a significant decrease in fetal body weight and length compared to the control group, along with an increase in these morphological abnormalities. Histopathological examination of the maternal liver revealed mild to moderate reversible damage. It can be concluded that high-dose andrographolide isolate has the potential to cause growth disturbances and fetal morphological abnormalities; thus, its use during pregnancy should be approached with caution.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Andrografolid, Fetus mencit, Histopatologi, Organogenesis, Teratogonik ------- Andrographolide, Fetal mice, Histopathology, Organogenesis, Teratogenic
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	01 Sep 2025 04:10
Last Modified:	01 Sep 2025 04:10
URI:	http://repository.stfi.ac.id/id/eprint/2749

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