PENAPISAN BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI SUSU SAPI MURNI

MAULIYA AMANI RAHMADINI, - (2025) PENAPISAN BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI SUSU SAPI MURNI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Biosurfaktan merupakan senyawa permukaan aktif yang dihasilkan oleh mikroorganisme, salah satunya bakteri asam laktat (BAL). BAL dapat diisolasi dari susu sapi murni yang memiliki populasi BAL terbanyak. Penelitian ini dilakukan untuk mengetahui potensi biosurfaktan dari isolat BAL hasil isolasi dari susu sapi murni. Isolasi dilakukan menggunakan media de Man, Rogosa, and Sharpe (MRS) agar. Identifikasi koloni bakteri dilakukan secara makroskopis dan mikroskopis menggunakan uji pewarnaan Gram uji katalase serta identifikasi molekuler menggunakan sekuensing gen 16S rRNA. Aktivitas biosurfaktan, diuji dengan metode drop collapse, indeks emulsifikasi (E24), dan oil spreading. Hasil isolasi diperoleh dua isolat BAL dengan kode BP-01 dan BP-02 yang menunjukkan bentuk koloni bulat berwarna putih dan katalase negatif. Kedua isolat berbentuk cocci (bulat) Gram positif dengan jenis diplo/tetrad pada isolat BP-01, dan jenis streptococci pada isolat BP-02 yang diduga termasuk genus Streptococcus. Analisis sekuensing gen 16S rRNA menunjukkan isolat BP-01 memiliki kemiripan tertinggi dengan Lactococcus lactis. Uji drop collapse dan oil spreading menunjukkan aktivitas positif biosurfaktan dengan terbentuknya tetesan datar dan zona bening pada minyak. Indeks emulsifikasi (E24) yang dihasilkan isolat BP-01 sebesar 58,21% dan isolat BP-02 sebesar 58,36%, menunjukkan kemampuan keduanya dalam menstabilkan emulsi. ------ Biosurfactants are surface-active compounds produced by microorganisms, one of which is lactic acid bacteria (LAB). LAB can be isolated from pure cow's milk, which has the largest LAB population. This study was conducted to determine the biosurfactant potential of LAB isolates isolated from pure cow's milk. Isolation was carried out using de Man, Rogosa, and Sharpe (MRS) agar media. Identification of bacterial colonies was carried out macroscopically and microscopically using the Gram staining test and catalase test, as well as molecular identification using 16S rRNA gene sequencing. Biosurfactant activity was tested using the drop collapse method, emulsification index (E24), and oil spreading. The isolation results obtained two LAB isolates with the code BP-01 and BP-02, which showed a white round colony shape and negative catalase. Both isolates were cocci (round) Grampositive with a diplo/tetrad type in isolate BP-01, and a streptococci type in isolate BP-02, which is suspected to belong to the genus Streptococcus. 16S rRNA gene sequencing analysis showed that isolate BP-01 had the highest similarity to Lactococcus lactis. Drop collapse and oil spreading tests demonstrated positive biosurfactant activity with the formation of flat droplets and clear zones in the oil. The emulsification index (E24) produced by isolate BP-01 was 58.21% and isolate BP-02 was 58.36%, indicating the ability of both to stabilize emulsions.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	biosurfaktan, bakteri asam laktat, susu sapi, emulsifikasi, 16S rRNA. ----- Biosurfactant, lactid acid bacteria, cow’s milk, emulsification, 16S rRNA.
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	01 Sep 2025 03:29
Last Modified:	01 Sep 2025 03:29
URI:	http://repository.stfi.ac.id/id/eprint/2746

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