DHIYA HIEROPHANY WAHDAH, - (2025) AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK MANGGIS, EKSTRAK KUNYIT, DAN EKSTRAK LADA PUTIH TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli, DAN Pseudomonas aeruginosa. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Larangan penggunaan Antibiotic Growth Promotor (AGP) pada pakan ternak berdasarkan regulasi pemerintah mendorong pencarian alternatif alami yang aman, murah, dan mudah diperoleh. Salah satu alternatif adalah pemanfaatan tanaman herbal yang mengandung metabolit sekunder dengan aktivitas antibakteri. Kulit manggis (Garcinia mangostana L.), kunyit (Curcuma domestica Val.), dan lada putih (Piper nigrum L.) mempunyai metabolit sekunder yang dapat menghambat pertumbuhan bakteri. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri, menentukan Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) ekstrak kulit manggis, kunyit, lada putih, dan kombinasinya terhadap Staphylococcus aureus, Escherichia coli, dan Pseudomonas aeruginosa secara in vitro. Uji KHM dilakukan dengan metode dilusi, sedangkan interaksi kombinasi ekstrak dengan metode difusi sumuran dan dinilai menggunakan Fractional Inhibitory Concentration Index (FICI). Kontrol positif yang digunakan adalah tetrasiklin, sedangkan kontrol negatif adalah DMSO. Hasil penelitian menunjukkan KHM ekstrak tunggal manggis, kunyit, dan lada putih masingmasing 6,25%, 3,125%, dan 12,5%. Kombinasi ekstrak dengan variasi perbandingan (1:3, 2:2, 3:1) menghasilkan KHM pada rentang 6,25–12,5%. Nilai FICI kombinasi adalah 2, kategori indifferent, yang menunjukkan kombinasi tidak memberikan peningkatan atau penurunan signifikan dibanding ekstrak tunggal. Meskipun demikian, hasil ini menunjukkan bahwa ekstrak tunggal maupun kombinasi tetap berpotensi digunakan sebagai feed additive alami pengganti AGP untuk mengendalikan bakteri patogen pada pakan ternak. ----- The ban on the use of Antibiotic Growth Promoters (AGP) in animal feed based on government regulations encourages the search for natural alternatives that are safe, inexpensive, and easily obtained. One alternative is the use of herbal plants containing secondary metabolites with antibacterial activity. Mangosteen peel (Garcinia mangostana L.), turmeric (Curcuma domestica Val.), and white pepper (Piper nigrum L.) have secondary metabolites that can inhibit bacterial growth. This study aims to evaluate the antibacterial activity, determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of extracts of mangosteen peel, turmeric, white pepper, and their combination against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in vitro. The MIC test was conducted using the dilution method, while the interaction of the extract combination was conducted using the well diffusion method and assessed using the Fractional Inhibitory Concentration Index (FICI). The positive control used was tetracycline, while the negative control was DMSO. The results showed that the MICs of single extracts of mangosteen, turmeric, and white pepper were 6.25%, 3.125%, and 12.5%, respectively. Combinations of extracts with varying ratios (1:3, 2:2, 3:1) produced MICs in the range of 6.25–12.5%. The FICI value of the combination was 2, in the indifferent category, indicating that the combination did not provide a significant increase or decrease compared to the single extracts. Nevertheless, these results indicate that both single extracts and combinations have the potential to be used as natural feed additives to replace AGPs to control pathogenic bacteria in livestock feed.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | antibakteri, ekstrak manggis, ekstrak kunyit, ekstrak lada putih, FICI. ----- antibacterial, mangosteen extract, turmeric extract, white pepper extract, FICI. |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 30 Aug 2025 02:18 |
| Last Modified: | 30 Aug 2025 02:18 |
| URI: | http://repository.stfi.ac.id/id/eprint/2727 |
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