REGINA YASMINE HERMAN, - (2025) STUDI IN SILICO DESAIN VAKSIN SUBUNIT PROTEIN DALAM UPAYA PENGEMBANGAN VAKSIN MONKEYPOX (MPOX). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Monkeypox (MPOX) merupakan penyakit zoonotik yang disebabkan oleh monkeypox virus (MPXV) dan masih menjadi ancaman kesehatan global. Pengembangan vaksin subunit dipandang sebagai pendekatan yang lebih aman dan efisien. Penelitian ini bertujuan merancang vaksin subunit protein fusi A35R-M1R menggunakan dua variasi linker fleksibel, yaitu (GGGGS)₂ dan (GGGGS)₃. Desain dilakukan secara in silico melalui pemodelan struktur 3D menggunakan AlphaFold, prediksi epitop sel B dengan Discotope 3.0, serta simulasi dinamika molekuler dengan perangkat lunak AMBER. Hasil menunjukkan bahwa desain vaksin dengan linker (GGGGS)₃ memiliki kestabilan struktural lebih tinggi dengan nilai RMSD dan RMSF yang lebih rendah dibandingkan varian (GGGGS)₂. Selain itu, prediksi epitop sel B menunjukkan distribusi yang lebih tersebar pada varian (GGGGS)₃. Simpulan dari penelitian ini adalah bahwa panjang linker berpengaruh terhadap kestabilan dan penyajian epitop dalam desain vaksin fusi, dan linker (GGGGS)₃ memberikan hasil yang lebih optimal. --- Monkeypox (mpox) is a zoonotic disease caused by the monkeypox virus (MPXV) and remains a global health threat. Subunit vaccine development is considered a safer and more efficient approach. This study aimed to design a subunit vaccine based on the fusion protein A35R-M1R using two variations of flexible linkers, namely (GGGGS)₂ and (GGGGS)₃. The design was performed in silico through 3D structural modeling using AlphaFold, B-cell epitope prediction via Discotope 3.0, and molecular dynamics simulation using the AMBER software. The results showed that the vaccine design with the (GGGGS)₃ linker demonstrated higher structural stability, indicated by lower RMSD and RMSF values compared to the (GGGGS)₂ variant. In addition, B-cell epitope prediction showed a broader epitope distribution in the (GGGGS)₃ variant. It was concluded that the linker length significantly affected the structural stability and epitope presentation of the vaccine construct, with (GGGGS)₃ yielding a more optimal outcome.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | In silico, MPOX, vaksin subunit, linker (GGGGS)n. ----- In silico, MPOX, subunit vaccine, linker (GGGGS)n |
| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 29 Aug 2025 02:47 |
| Last Modified: | 29 Aug 2025 02:47 |
| URI: | http://repository.stfi.ac.id/id/eprint/2714 |
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