SELVI NURAINI, - (2023) ANALISIS KADAR ANDROGRAFOLID DARI TANAMAN SAMBILOTO (Andrographis paniculata (Burm.f) Nees) PADA SAMPEL DARAH MANUSIA MENGGUNAKAN METODE KROMATOGRAFI CAIR KINERJA TINGGI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Sambiloto adalah tanaman yang sering dimanfaatkan sebagai obat tradisional di Indonesia, yang mengandung senyawa andrografolid. Andrografolid memiliki aktivitas farmakologi seperti antiinflamasi, antikanker, hepatoprotektor, antimalaria, antioksidan, antidiabetes, antihiperlipidemia, dan antivirus. Penelitian ini bertujuan untuk mengetahui metode analisis dan mengetahui pengaruh matriks darah terhadap kadar isolat andrografolid menggunakan metode Kromatografi Cair Kinerja Tinggi (KCKT). Pada penelitian ini, andrografolid diekstraksi dalam darah dengan metode pengendapan protein menggunakan metanol yang divortex selama 30 detik, dan disentrifugasi pada kecepatan 3000 rpm selama 15 menit. Analisis dilakukan dengan isokratik pada kolom C18 (Zobrax Eclipse Plus 4.6 x 150 mm) dan fase gerak metanol-air (65:35v/v) pada laju alir 1,0 mL/menit. Deteksi dilakukan pada panjang gelombang 223 nm dan volume penyuntikan 10 µL. Pada rentang konsentrasi 20-120 ppm dihasilkan kurva kalibrasi yang linear dengan (R2) sebesar 0,9922. Nilai %recovery berada direntang 99,21-105,23% dengan %RSD berada pada rentang 0,49-2,33%. LOD berada pada konsentrasi 12,21 ppm dan LOQ 37,01 ppm. Rata-rata kadar andrografolid dalam sampel sebesar 75,80%. Dengan demikian, metode KCKT dapat digunakan untuk analisis kadar isolat andrografolid dalam sampel darah manusia dan terdapat pengaruh matriks darah terhadap kadar isolat andrografolid. --- Sambiloto is a plant that is commonly utilized as traditional medicine in Indonesia, containing the compound andrographolide. Andrographolide exhibits pharmacological activities such as anti-inflammatory, anticancer, hepatoprotective, antimalarial, antioxidant, antidiabetic, anti-hyperlipidemia, and antiviral effects. This study aims to determine the analysis method and understand the influence of blood matrix on the level of isolated andrographolide using High Performance Liquid Chromatography (HPLC). In this research, andrographolide is extracted from blood using a protein precipitation method with methanol, which is vortexed for 30 seconds and centrifuged at a speed of 3000 rpm for 15 minutes. The analysis is performed isocratically on a C18 column (Zorbax Eclipse Plus 4.6 x 150 mm) with a mobile phase of methanol-water (65:35v/v) at a flow rate of 1.0 mL/minute. Detection is conducted at a wavelength of 223 nm with an injection volume of 10 µL. Within the concentration range of 20-120 ppm, a linear calibration curve is generated with an R-squared (R2) value of 0.9922. The %recovery values range from 99.21% to 105.23%, with %RSD in the range of 0.49% to 2.33%. The Limit of Detection (LOD) is at a concentration of 12.21 ppm and the Limit of Quantitation (LOQ) is 37.01 ppm. The average content of andrographolide in the samples is 75.80%.Thus, the HPLC method can be employed for the analysis of isolated andrographolide levels in human blood samples, and there is an influence of the blood matrix on the level of isolated andrographolide.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Andrografolid, isolat, matriks dalam sampel darah, Kromatografi Cair Kinerja Tinggi (KCKT). Andrographolide, isolated, blood sample matrix, High-Performance Liquid Chromatography (HPLC). |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 19 Jun 2024 07:42 |
| Last Modified: | 19 Jun 2024 07:42 |
| URI: | http://repository.stfi.ac.id/id/eprint/233 |
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