AURI SUBARTA, - (2023) UJI TOKSISITAS AKUT ORAL ISOLAT ANDROGRAFOLID TERHADAP TIKUS UJI BETINA DENGAN METODE OECD 425 UP AND DOWN PROCEDURE. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Pemanfaatan obat bahan alam Indonesia yang terjamin mutu, khasiat, dan keamanannya untuk meningkatkan derajat kesehatan masyarakat semakin tumbuh dan berkembang saat ini. Penelitian ini bertujuan untuk mengevaluasi toksisitas akut oral isolat andrografolid terhadap tikus uji betina menggunakan metode OECD 425 Up and Down Procedure. Tikus uji betina yang sehat dan dewasa dibagi menjadi dua kelompok dengan dosis yang sama dari isolat andrografolid dengan dosis 2000 mg/kgBB yang diberikan secara oral. Metode OECD 425 Up and Down Procedure digunakan untuk menentukan LD₅₀ dan efek toksik akut pada tikus uji. Dosis awal ditentukan berdasarkan perhitungan dan tinjauan literatur sebelumnya. Setelah pemberian dosis awal, pengamatan dilakukan terhadap tikus uji selama periode waktu yang ditentukan, dengan pemantauan gejala klinis, berat badan, dan tingkat mortalitas, serta pengamatan yang terjadi pada organ lambung, hati, ginjal dan limpa. Data yang diperoleh dianalisis menggunakan software AOT425StatPgm (Acute Oral Toxicity Guideline 425 Statistical Programme). Hasil penelitian menunjukkan bahwa isolat andrografolid tidak menunjukkan efek toksisitas akut pada tikus uji betina. Hasil skoring pada histopatologi organ menunjukkan adanya erosi mukosa pada lambung, degenerasi dan nekrosis pada hati serta ginjal. Berdasarkan data dan pengamatan yang diperoleh termasuk dalam kategori tidak toksik berdasarkan nilai dosis terbatas pada dosis 2000 mg/kgBB, namun demikian harus tetap mempertimbangkan gejala kelainan pada organ lambung, hati, ginjal dan limpa. Studi lanjut diperlukan untuk lebih memahami mekanisme dan dampak toksisitas andrografolid pada organisme lain serta untuk mengevaluasi risiko penggunaannya pada manusia. --- Currently, the use of Indonesian natural medicine with guaranteed quality, efficacy, and safety to improve public health status is growing and developing. This study aims to evaluated the acute oral toxicity of andrographolide isolate in female rats using the OECD 425 Up and Down Procedure. Healthy and adult female test rats were divided into two groups and with the same dose of andrographolide isolate at a dose of 2000 mg/kg BW given orally. The OECD 425 Up and Down Procedure method was used to determine the dose of LD₅₀ and acute toxic effects in test rats. The initial dose was determined based on previous calculations and literature review. After administration of the initial dose, observations were made on the test rats for a specified period of time by monitoring clinical symptoms, body weight, and mortality rates, as well as observations that occurred in the organs of the stomach, liver, kidneys, and spleen. The data obtained were analyzed using AOT425StatPgm software (Acute Oral Toxicity Guideline 425 Statistical Program). The results showed that andrographolide isolates did not show acute toxicity effects on female rats. The results of the observations showed that the scoring results on the histopathology of the organs showed mucosal erosion in the stomach, degeneration, and necrosis in the liver and kidneys. Based on the data and observations obtained, andrographolide isolate is included in the non-toxic category based on the limit dose value at a dose of 2000 mg/kg BW, however, symptoms of abnormalities in the stomach, liver, kidney, and spleen must still be considered. it is recommended for its use in the pharmaceutical field. Further studies are needed to better understand the mechanisms and effects of andrographolide toxicity in other organisms, as well as to evaluate the risks of its use in humans.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Andrografolid, dosis, letal dose 50, OECD-425, toksisitas. andrographolide, dose, letal dose 50, OECD-425, toxicity. |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 14 Jun 2024 07:00 |
| Last Modified: | 26 Jun 2024 01:30 |
| URI: | http://repository.stfi.ac.id/id/eprint/210 |
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