DEYA PUTRI RAMADHANI, - (2023) ANALISIS SEKUENSING SERTA KONSTRUKSI GEN srfAA, srfAB, srfAC DARI BAKTERI Staphylococcus epidermidis PADA PLASMID pUC57 DAN pPM ,SERTA DARI BAKTERI Bacillus cereus PADA PLASMID pUC57 DAN pBT6. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Salah satu biosurfaktan golongan lipopeptida yaitu Surfactine synthase, kelompok lipopeptida yang paling popular. Surfactine synthase memiliki sifat bioaktif yang diproduksi oleh beberapa bakteri seperti Bacillus cereus dan bakteri Staphylococcus epidermidis. Surfactine synthase digunakan dalam penelitian ini sebagai gen target untuk dilakukan analisis sekuensing dari gen surfactine synthase yang bertujuan untuk pengembangan gen surfactin synthase dari bakteri Bacillus cereus dan selanjutnya Mencari enzim restriksi yang tepat untuk melakukan konstruksi gen surfactine synthase dari bakteri Bacillus cereus terhadap plasmid pUC57 dan pBT6 serta bakteri Staphylococcus epidermidis pada plasmid pUC57 dan pPM. analisis sekuensing menunjukkan sekuence sampel memiliki kemiripan Panjang sekuens sebesar 74% dan kesamaan basa nukleotida sebesar 99,13% dengan gen Surfactine synthase dari bakeri Bacillus cereus dibandingkan bakteri Staphylococcus epidermidis. sekuence ini kemudian dilanjutkan dengan analisis penentuan enzim restriksi yang tidak memotong sekuence pada vector plasmid. Didapat enzim restriksi yang sesuai adalah EcoRI dan BamHI. Hasil konstruksi gen pada plasmid pUC57 adalah pada posisi bp 167 dan Kodon stop ditemukan di dalam CDS pada posisi bp 167. Penelitian ini dapat memberikan inovasi teknologi atau pengembangan baru untuk menghasilkan gen Surfactine synthase dari bakteri Bacillus cereus. --- One of the lipopeptide biosurfactants is Surfactine synthase, the most popular lipopeptide group. Surfactine synthase has bioactive properties which are produced by several bacteria such as Bacillus cereus and Staphylococcus epidermidis bacteria. Surfactine synthase was used in this study as a target gene for analysis of the sequencing of the surfactine synthase gene with the aim of developing the surfactin synthase gene from the bacterium Bacillus cereus and then looking for the right restriction enzymes to carry out the construction of the surfactine synthase gene from the bacterium Bacillus cereus against plasmids pUC57 and pBT6 and Staphylococcus epidermidis on the plasmid pUC57 and pPM. Sequencing analysis showed that the sample sequences had 74% similarity in length and 99.13% similarity in nucleotide bases with the Surfactine synthase gene from Bacillus cereus bacteria compared to Staphylococcus epidermidis. This sequence is then followed by analysis of restriction enzyme release that does not cut the sequence in the plasmid vector. The suitable restriction enzymes were EcoRI and BamHI. The result of gene construction on the pUC57 plasmid is at position bp 167 and a stop codon is found in CDS at position bp 167. This research can provide technological innovation or new development to produce the Surfactine synthase gene from Bacillus cereus bacteria.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Surfactine synthase, pUC57, lipopeptida, Bacillus cereus, DNA rekombinan. Surfactin synthase, pUC57, lipopeptide, Bacillus cereus, recombinant DNA |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 07 Jun 2024 07:38 |
| Last Modified: | 07 Jun 2024 07:38 |
| URI: | http://repository.stfi.ac.id/id/eprint/190 |
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