IRMA IRWANDA, - (2023) VALIDASI METODE PENETAPAN KADAR ISOLAT ANDROGRAFOLID DARI TANAMAN SAMBILOTO (Andrographis paniculata (Burm.f.) Ness) DALAM SAMPEL PLASMA SECARA IN VITRO MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI – ULTRAVIOLET. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Andrografolid merupakan kandungan utama yang terdapat dalam sambiloto (Andrographis paniculata (Burm.f.) Ness) yang mempunyai berbagai aktivitas farmakologis seperti antioksidan dan antiinflamasi. Obat erat kaitannya dengan efektivitas obat didalam tubuh dan salah satu parameternya adalah penetapan kadar obat. Tujuan penelitian ini dilakukan optimasi kondisi analisis dan validasi untuk menentukan metode penetapan kadar Andrografolid dalam plasma darah dengan menggunakan metode. Kromatografi Cair Kinerja Tinggi (KCKT). Andrografolid diekstraksi dari plasma dengan pengendapan protein menggunakan metanol. Metanol dicampurkan ke dalam plasma dengan perbandingan 1:1 kemudian dikocok menggunakan vortex selama 20 detik, dan disentrifugasi pada kecepatan 3000 selama 10 menit. Sistem kromatografi terdiri dari kolom Zorbax Eclipse C18 (4,6 x 150mm, 5µm), fase gerak metanol dan air dengan perbandingan 65:35 v/v; laju alir 1 mL/menit, deteksi pada panjang gelombang 223 nm. Hasil dari penelitian yang telah dilakukan ini menunjukkan linieritas pada rentang konsentrasi 0,5-25 ppm dengan nilai koefisien korelasi (r) 0,989 dengan batas kuantitasi 11,535 μg/mL dan batas deteksi 3,086 μg/mL. Uji Akurasi (%Recovery berada direntang 107,78-112,71) dan presisi (intra hari dan inter hari dengan % RSD ≤ 15% (kecuali pada konsentrasi 1 ppm). Berdasarkan penelitian ini, dapat disimpulkan bahwa Andrografolid dalam plasma dapat dianalisis menggunakan KCKT. --- Andrografolid is the main content found in sambiloto (Andrographis paniculata (Burm.f.) Ness) which has various pharmacological activities such as antioxidants and anti-inflammatory. Drug development is closely related to the effectiveness of drugs in the body and one of the parameters is the determination of drug levels in plasma. The purpose of this study was to optimize analytical conditions and validation to determine the method of determining Andrografolid levels in blood plasma using the High Performanc Liquid Chromatography (HPLC) method. Andrografolid was extracted from plasma by protein precipitation using methanol. Methanol was mixed into plasma in a ratio of 1:1 then shaken using a vortex for 20 seconds, and centrifuged at 3000 speed for 10 minutes. The chromatography system consisted of a Zorbax Eclipse C18 column (4.6 x 150mm, 5µm), a mobile phase of methanol and water with a ratio of 65:35 v/v; flow rate of 1 mL/min, detection at a wavelength of 223 nm. The results of this research showed linearity in the concentration range of 0.5-25 ppm with a correlation coefficient (r) of 0.989 with a limit of quantitation of 3.336 μg/mL and a limit of detection of 1.310 μg/mL. Accuracy test (%Recovery is in the range of 107.78-112.71) and precision (intra-day and inter-day with % RSD ≤ 15% (except at a concentration of 1 ppm). Based on this study, it can be concluded that Andrografolid in plasma can be analyzed using KCKT.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Andrografolid, KCKT, validasi metode Andrographolide, KCKT, validation method |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 06 Jun 2024 08:06 |
| Last Modified: | 06 Jun 2024 08:06 |
| URI: | http://repository.stfi.ac.id/id/eprint/183 |
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