EFEKTIVITAS KONDISI SUHU ANNEALING PCR GEN FENGYCIN DAN ITURIN PADA BAKTERI Bacillus cereus

FEGI ABRIYANTO, - (2023) EFEKTIVITAS KONDISI SUHU ANNEALING PCR GEN FENGYCIN DAN ITURIN PADA BAKTERI Bacillus cereus. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Biosurfaktan merupakan senyawa permukaan aktif yang berasal dari mikroorganisme yang memiliki sifat biodegradable, tidak berbahaya dan menggunakan sumber yang dapat diperbaharui. Bakteri Bacillus cereus berpotensi sebagai penghasil biosurfaktan golongan lipopeptida. Penelitian ini dilakukan untuk mengisolasi gen pengkode enzim fengycin dan iturin dari Bacillus cereus menggunakan metode Polymerase Chain Reaction (PCR). Genom Bacillus cereus diisolasi menggunakan kit ekstraksi DNA dan dianalisis menggunakan gel elektroforesis agarose. Gen fengycin dan iturin diisolasi dengan mengamplifikasi genom menggunakan primer spesifik pada variasi suhu annealing. Keberadaan fragmen DNA dianalisis menggunakan elektroforesis gel agarose. Hasil isolasi genom menunjukan terdapat pita dengan ukuran lebih dari 10 kb. Hasil elektroforesis ekstraksi genom menunjukkan terdapat pita dengan ukuran lebih dari 10 kb. Hasil elektroforesis dari PCR primer fengycin fen BC dengan suhu annealing 61°C terdapat pita berukuran 0,25 kb dan fen CN dengan suhu annealing 66°C terdapat pita berukuran 0,5 kb sedangkan gen iturin srfA dengan suhu annealing 57°C terdapat pita berukuran 5 kb sedangkan primer gen iturin srfB dengan suhu annealing 57°C terdapat pita berukuran 4 kb. Menunjukan bahwa gen yang teramplifikasi diduga gen fengycin dan iturin karena ukurannya tidak lebih dari 10 kb. --- Biosurfactants are surface active compounds derived from microorganisms that are biodegradable, harmless and use renewable sources. Bacillus cereus bacteria has the potential to produce lipopeptide class of biosurfactants. This research was conducted to isolate the genes coding for the enzymes fengycin and iturin from Bacillus cereus using the Polymerase Chain Reaction (PCR) method. The genome of Bacillus cereus was isolated using a DNA extraction kit and analyzed using agarose gel electrophoresis. The fengycin and iturin genes were isolated by amplifying the genome using specific primers at various annealing temperatures. The presence of DNA fragments was analyzed using agarose gel electrophoresis. The genome isolation results show that there are bands with a size of more than 10 kb. The results of genome extraction electrophoresis showed that there were bands with a size of more than 10 kb. The electrophoresis results from the PCR primer fengycin fen BC with an annealing temperature of 61°C had a band of 0.25 kb and fen CN with an annealing temperature of 66°C had a band of 0.5 kb while the iturin srfA gene with an annealing temperature of 57°C had a band of 5 kb while the primer of the iturin srfB gene with an annealing temperature of 57°C has a 4 kb band. It shows that the amplified genes are thought to be the fengycin and iturin genes because their size is not more than 10 kb.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Ekstraksi DNA, PCR, Elektroforesis. DNA Extraction, Polymerase Chain Reaction, Electrophoresis.
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	06 Jun 2024 06:13
Last Modified:	06 Jun 2024 06:13
URI:	http://repository.stfi.ac.id/id/eprint/176

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