NADIANA ANTIARA, - (2024) UJI AKTIVITAS PENGHAMBATAN ENZIM HMG COA REDUKTASE DAN LIPASE MENGGUNAKAN EKSTRAK DAN FRAKSI DAUN CINCAU HITAM (Mesona palustris BL). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Daun cincau hitam telah lama digunakan dalam makanan dan minuman tradisional serta sebagai obat herbal dengan khasiat antioksidan, antiinflamasi, dan antihiperkolesterol. Hiperkolesterolemia, ditandai dengan kadar kolesterol tinggi, dapat meningkatkan risiko penyakit jantung dan stroke. Penyebabnya termasuk penumpukan asam lemak bebas dan produksi kolesterol berlebih. Penghambatan enzim lipase dan HMG-CoA reduktase dapat menurunkan kadar kolesterol. Tujuan penelitian ini adalah untuk mengetahui aktivitas penghambatan kerja enzim lipase dan HMGCoA reduktase menggunakan ekstrak dan fraksi daun cincau hitam. Ekstraksi daun cincau hitam menggunakan metode maserasi dengan pelarut etanol 96%. Penelitian ini dilakukan secara in vitro menggunakan metode ELISA. Hasil pengujian aktivitas penghambatan enzim HMG-CoA reduktase menunjukkan bahwa ekstrak etanol dan fraksi daun cincau hitam berpotensi menghambat enzim HMG CoA dan lipase. Ekstrak etanol menunjukkan penghambatan tertinggi terhadap enzim HMG-CoA reduktase dengan nilai 75,96% dan aktivitas enzim 0,183 mU/mL, sedangkan fraksi air daun cincau hitam menghambat enzim lipase dengan nilai 97,33% dan aktivitas enzim 0,829 mU/mL. Secara statistik menunjukan bahwa tidak ada perbedaan yang signifikan dengan nilai (p>0,05). Hal ini menunjukkan bahwa daun cincau hitam berpotensi sebagai antihiperkolesterol. ----- Black grass jelly leaves have long been used in traditional foods and drinks as well as as a herbal medicine with antioxidant, anti-inflammatory and antihypercholesterol properties. Hypercholesterolemia, characterized by high cholesterol levels, can increase the risk of heart disease and stroke. Causes include a buildup of free fatty acids and excess cholesterol production. Inhibition of lipase and HMG-CoA reductase enzymes can reduce cholesterol levels. The aim of this research was to determine the inhibitory activity of lipase and HMG-CoA reductase enzymes using extracts and fractions of black grass jelly leaves. Black grass jelly leaves were extracted using the maceration method with 96% ethanol solvent. This research was carried out in vitro using the ELISA method. The results of testing the inhibitory activity of the HMG-CoA reductase enzyme showed that the ethanol extract and black grass jelly leaf fraction had the potential to inhibit the HMG CoA and lipase enzymes. The ethanol extract showed the highest inhibition of the HMGCoA reductase enzyme with a value of 75.96% and an enzyme activity of 0.183 mU/mL, while the water fraction of black grass jelly leaves inhibited the lipase enzyme with a value of 97.33% and an enzyme activity of 0.829 mU/mL. Statistically it shows that there is no significant difference in value (p>0.05). This shows that black grass jelly leaves have the potential to act as antihypercholesterol.
| Item Type: | Thesis (Skripsi) |
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| Uncontrolled Keywords: | Daun Cincau Hitam, Enzim HMG CoA Reduktase, Enzim Lipase, ELISA, Antihiperkolesterol. ---- Black Grass jelly Ext, HMG CoA Reductase, Lipase, ELISA, Antihypercholesterol |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 13 Nov 2024 07:14 |
| Last Modified: | 13 Nov 2024 07:14 |
| URI: | http://repository.stfi.ac.id/id/eprint/1481 |
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