UJI TOKSISITAS SUB-KRONIS ORAL ISOLAT ANDROGRAFOLID DARI ASPEK BIOKIMIA PADA MENCIT (Mus musculus L.) GALUR Swiss Webster MENGGUNAKAN METODE OECD 407

AQSHAL HABAL SAFAAT, - (2024) UJI TOKSISITAS SUB-KRONIS ORAL ISOLAT ANDROGRAFOLID DARI ASPEK BIOKIMIA PADA MENCIT (Mus musculus L.) GALUR Swiss Webster MENGGUNAKAN METODE OECD 407. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Isolat andrografolid menunjukkan berbagai aktivitas farmakologi, diantaranya antihiperglikemia, antihiperlipidemia, dan efek hepatoprotektif. Andrografolid memiliki potensi untuk digunakan sebagai sediaan obat namun harus mempertimbangkan faktor keamanan jika digunakan berulang. Penelitian ini bertujuan untuk mengevaluasi efek toksisitas subkronis isolat andrografolid dari aspek biokimia pada mencit galur Swiss Webster. Uji toksisitas dilakukan dengan menggunakan metode OECD 407 dengan pemberian beragam dosis, dimulai dosis kecil 5 mg, dosis menengah 500 mg dan dosis tinggi 1000 mg/KgBB, dosis satelit 1000 mg/KgBB, disertai kontrol normal dan kontrol satelit. Hewan uji yang digunakan adalah mencit jantan dan betina, masing-masing 30 ekor. Kajian biokimia yang diamati, yaitu kadar glukosa, kolesterol total, AST (aspartat aminotransferase), dan kreatinin dalam plasma darah. Hasil penelitian menunjukkan bahwa pemberian isolat andrografolid selama 28 hari tidak menimbulkan gejala toksik yang signifikan, dan kadar biokimia hewan uji tidak menunjukan perbedaan signifikan dibandingkan kelompok kontrol normal; begitu juga pada kelompok satelit. Penurunan kadar glukosa terjadi pada dosis 500 mg/kgBB dan 1000 mg/kgBB walaupun masih dalam rentang normal; kadar AST meningkat namun juga masih dalam batas normal. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian isolat andrografolid selama 28 hari pada dosis 5 mg, 500 mg dan 100 mg masih dapat dikatakan aman, namun demikian perlu kehati-hatian terhadap penurunan kadar glukosa pada pemberian jangka panjang. ---- Andrografolid isolate shows various pharmacological activities including antihyperglycemia, anti-hyperlipidemia, and hepatoprotective effects. Andrografolid has the potential to be used as medical preparation but safety factors must be considered if used repeatedly. This study aims to evaluate the subchronic toxicity effects of andrografolid isolates from biochemical aspects on Swiss Webster mice. Toxicity tests were carried out using the OECD 407 method by administering various doses, starting with a small dose of 5 mg, a medium dose of 500 mg and a high dose of 1000 mg/KgBW, a satelit dose of 1000 kg/BW, acommpained by normal controls and satellite controls. The test animals used were male and female mice, 30 each. Biochemical studies were observed, namely levels of glucose, total cholesterol, AST (aspartate amino-transferase), and creatinine in bloods plasma. The results showed that the administration of andrografolid isolate for 28 days did not cause significant toxic symptoms, and biochemical levels of the test animals did not show significant differences compared to the normal control group; likewise for the Satellite group. A decrease in glucose levels occurred ate a dose of 500 mg/kgBW and dose of 1000 mg/kgBW even thought it was still within the normal range; AST levels increased but where still within normal limits. Based on the riset results, it can be concluded that the administering andrografolid isolate for 28 days at doses of 5 mg, 500 mg and 1000 mg can still be said to be safe, however, care needs to be taked regarding decreasing glucose levels during long-trem administrasion.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	andrografolid, subkronis, biokimia, OECD-407. ---- andrographolid, subchronic, biochemical, OECD-407.
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	12 Nov 2024 08:16
Last Modified:	12 Nov 2024 08:16
URI:	http://repository.stfi.ac.id/id/eprint/1467

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