YOSEDIEN LUTFIA KUSUMASTUTI, - (2020) UJI AKTIVITAS ANTIBAKTERI SARI BUAH NAGA MERAH (Hylocereus polyrhizus) TERFERMENTASI TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Diare merupakan penyakit infeksi pada saluran pencernaan yang dapat disebabkan oleh bakteri Escherichia coli dan Staphylococcus aureus. Konsumsi minuman probiotik dapat menjadi alternatif dalam mengatasi gangguan pencernaan. Tujuan penelitian adalah mengetahui aktivitas antibakteri dari sari buah naga yang difermentasi oleh Lactobacillus plantarum terhadap bakteri Escherichia coli dan Staphylococcus aureus. Proses pembuatan sari buah naga terfermentasi dilakukan dengan penambahan bakteri Lactobacillus plantarum konsentrasi 0%; 1%; 2%; 3%; 4%; 5% dan diinkubasi selama 24 jam. Evaluasi produk meliputi organoleptis, pH, dan total bakteri asam laktat. Pengujian antibakteri dilakukan dengan metode sumuran. Hasil organoleptis menunjukkan bahwa sampel yang disukai ialah sampel dengan konsentrasi 1%. Hasil pH menunjukkan bahwa sampel dengan konsentrasi 1% memiliki pH yang sesuai dengan literatur yaitu 4,31. Hasil total bakteri asam laktat menunjukkan bahwa semua konsentrasi memenuhi standar SNI yaitu 107 cfu/mL. Hasil uji KHM yang sudah dilakukan, didapatkan hasil untuk konsentrasi 4% dengan diameter 2,9mm untuk bakteri Eschericia coli dan 4mm untuk bakteri Staphylococcus aureus dikategorikan aktivitas hambatnya lemah. ---- Diarrhea is an infectious disease of the digestive tract that can be caused by the bacteria Escherichia coli and Staphylococcus aureus. Consumption of probiotic drinks can be an alternative in overcoming digestive disorders. The research objective was to determine the antibacterial activity of dragon fruit juice fermented by Lactobacillus plantarum against Escherichia coli and Staphylococcus aureus bacteria. The process of making fermented dragon fruit juice was carried out by adding 0% Lactobacillus plantarum bacteria; 1%; 2%; 3%; 4%; 5% and incubated for 24 hours. The product evaluation includes organoleptic, pH, and total lactic acid bacteria. Antibacterial testing is done by using the well method. Organoleptic results show that the preferred sample is a sample with a concentration of 1%. The pH results show that the sample with a concentration of 1% has a pH in accordance with the literature, namely 4,31. The total results of lactic acid bacteria showed that all concentrations met the SNI standard, namely 107 cfu / ml. The results of the MIC test that have been carried out, obtained results for a concentration of 4% with a diameter of 2,9 mm for Escherichia coli bacteria and 4 mm f
| Item Type: | Thesis (Skripsi) |
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| Uncontrolled Keywords: | Buah Naga, Fermentasi, Metode sumuran, Escherichia coli, Staphylococcus aureus ---- Dragon Fruit, Fermentation, Well method, Escherichia coli, Staphylococcus aureus |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 02 Oct 2024 02:38 |
| Last Modified: | 02 Oct 2024 02:38 |
| URI: | http://repository.stfi.ac.id/id/eprint/1383 |
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