SHELA AGNES ABRAHAM, - (2020) PENENTUAN SIKLUS MAKSIMUM PENGGUNAAN KROMATOGRAFI PENUKAR ION DENGAN FASA DIAM RESIN DEAE PADA TAHAP PEMURNIAN KRUD HBsAg PRODUKSI PT. BIOFARMA. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
HBV (Hepatitis B Virus) merupakan salah satu penyakit yang mematikan. Infeksi Hepatitis B dapat dicegah dengan vaksin rekombinan Hepatitis B. Tujuan penelitian ini adalah menentukan siklus maksimal penggunaan kromatografi penukar ion dengan fasa diam resin DEAE (Diethylaminoethyl) dalam pemurnian krud HBsAg produksi PT. Biofarma untuk produksi skala besar vaksin HBV. Optimasi metode dilakukan pada tahap kromatografi, sehingga mendapatkan pemisahan yang baik dan yield yang tinggi. Uji Lowry dan uji kadar protein HPLC (High Performance Liquid Chromatography) UV 280 nm dilakukan untuk melihat jumlah protein setiap siklusnya. Hasil siklus maksimum kromatografi penukar ion yang didapatkan adalah 16 siklus. Uji Lowry dan dan uji kadar protein HPLC UV 280 nm memiliki kesamaan yaitu semakin banyak siklus, jumlah protein semakin menurun. Jumlah protein menurun kurang lebih sekitar 24,28%. ---- HBV (Hepatitis B Virus) is one of deadly disease. Hepatitis B infection can be prevented by recombinant Hepatitis B vaccine. The aim of this research is to determine the maximum cycles of ion exchange chromatography with DEAE resin (Diethylaminoethyl) as stationary phase in the purification of HBsAg crude which produced by PT. Biofarma for large-scale production of recombinant HBV vaccines. The optimization of the method is carried out at the chromatography stage, to obtain good separation and high yield of protein target. Lowry test and HPLC (High Performance Liquid Chromatography) UV 280 nm protein content test were carried out to see the amount of protein for each cycle. The maximum cycles of ion exchange chromatography was 16 cycles. The result from Lowry's test and and HPLC protein content test have in common, the more cycles, the lower the protein amount. The amount of protein decreased by approximately 24.28%.
| Item Type: | Thesis (Skripsi) |
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| Uncontrolled Keywords: | Vaksin HBV, HBsAg, kromatografi penukar ion, uji Lowry. ---- HBV vaccine, HBsAg, ion exchange chromatography, Lowry’s test |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 02 Oct 2024 03:10 |
| Last Modified: | 02 Oct 2024 03:10 |
| URI: | http://repository.stfi.ac.id/id/eprint/1361 |
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