RAJA ADHEA AMELDA ARAFAH, - (2020) PENINGKATAN KADAR FLAVONOID MELALUI PENAMBAHAN ELISITOR KOMBINASI ANTARA KITOSAN DAN ASAM SALISILAT PADA KULTUR TANAMAN TIN (Ficus carica L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Tanaman tin (Ficus carica L.) merupakan salah satu tanaman yang memproduksi flavonoid. Elisitor merupakan senyawa yang diberikan pada kadar kecil pada sistem sel hidup untuk meningkatkan biosintesis senyawa-senyawa tertentu, contohnya flavonoid sehingga elisitor kombinasi antara kitosan dan asam salisilat yang ditambahkan bertujuan untuk meningkatkan kadar total flavonoid dalam tanaman tin. Kultur tanaman tin menggunakan media BAP (Benziyl Amino Purin) 1 ppm dan NAA (Naphthalena Acetid Acid) 0,1 ppm. Penambahan kombinasi elisitor dilakukan pada hari ke-28 dengan variasi konsentrasi, yaitu kitosan 0,2 mg/mL dan asam salisilat 0,05 mmol (Ka), kitosan 0,2 mg/mL dan asam salisilat 0,06 mmol (Kb), kitosan 0,3 mg/mL dan asam salisilat 0,05 mmol (Kc), serta kitosan 0,3 mg/mL dan asam salisilat 0,06 mmol (Kd). Hari ke-30 dipanen dan diekstrasi dengan etanol 96% serta dilakukan skrining fitokimia. Ekstrak etanol 96% digunakan untuk pengecekkan kadar total flavonoid menggunakan sepektrofotometer UV-Visible. Hasil dibandingkan dengan planlet yang tidak menggunakan kombinasi elisitor menunjukkan bahwa adanya peningkatan kadar total flavonoid yang mana pada konsentrasi Ka kadar total flavonoidnya sebanyak 0,917g/100g ekstrak, Kb 3,367g/100g ekstrak, Kc 0,797g/100g ekstrak dan Kd 1,433g/100g ekstrak. Kadar flavonoid yang tertinggi pada ekstrak etanol 96% dengan konsentrasi elisitor kombinasi i kitosan 0,2 mg/mL dan asam salisilat 0,06 mmol, yaitu 3,367g/100g ekstrak. --- Fig ara (Ficus carica L.) is a plant that produces flavonoids. Elicitors are compounds that given at small levels in living cell systems to increase the biosynthesis of certain compounds, for example flavonoids that the combined elicitor between chitosan and salicylic acid is added to increase the total levels of flavonoids in tin plants. Tin plant culture used BAP (Benziyl Amino Purin) 1 ppm and NAA (Naphthalena Acetid Acid) 0.1 ppm for media. The addition of the elicitor combination was carried out on the 28th day with various concentrations, namely 0.2 mg/mL chitosan and 0.05 mmol salicylic acid, 0.2 mg/mL chitosan and 0.06 mmol salicylic acid, chitosan 0.3 mg/mL and salicylic acid 0.05 mmol, chitosan 0.3 mg/mL and salicylic acid 0.06 mmol. Day 30 was harvested and extracted with 96% ethanol and phytochemical screening was carried out. 96% ethanol extract was used to check the total levels of flavonoids using a spectrophotometer UV-Visible. The results compared to the plantlets that did not use the elicitor combination showed that there was an increase in total flavonoid levels, the total flavonoid level was 0.917g/100g extract, 3.367g/100g extract, 0.797g/100g extract and 1.433g/100g extract. The highest level of flavonoids was in the 96% ethanol extract with a combined elicitor concentration of chitosan 0.2 mg /mL and 0.06 mmol of salicylic acid, which is 3.367g / 100g of extract.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Kultur tanaman, elisitor, Ficus carica L., flavonoid. --- Plant tissue, elicitor, Ficus carica L., flavonoid |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 04 Oct 2024 01:22 |
| Last Modified: | 04 Oct 2024 01:22 |
| URI: | http://repository.stfi.ac.id/id/eprint/1347 |
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