LOKASI GEN BIOSURFAKTAN PADA BAKTERI Bacillus cereus DAN Brevundimonas terrae MENGGUNAKAN METODE BIOINFORMATIKA

MAULANA ILHAM MUNAJAT, - (2020) LOKASI GEN BIOSURFAKTAN PADA BAKTERI Bacillus cereus DAN Brevundimonas terrae MENGGUNAKAN METODE BIOINFORMATIKA. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

[img] Text
S_PSSF_A162034_Title.pdf
Download (767kB)
[img] Text
S_PSSF_A162034_Chapter1.pdf
Restricted to Repository staff only
Download (94kB)
[img] Text
S_PSSF_A162034_Chapter2.pdf
Restricted to Repository staff only
Download (735kB)
[img] Text
S_PSSF_A162034_Chapter3.pdf
Restricted to Repository staff only
Download (1MB)
[img] Text
S_PSSF_A162034_Chapter4.pdf
Restricted to Repository staff only
Download (2MB)
[img] Text
S_PSSF_A162034_Chapter5.pdf
Restricted to Repository staff only
Download (87kB)
[img] Text
S_PSSF_A162034_Appendix.pdf
Restricted to Repository staff only
Download (2MB)
Official URL: https://repository.stfi.ac.id/

Abstract

Biosurfaktan merupakan alternatif surfaktan sintesis yang disintesis secara ekstraseluler oleh mikroorganisme yang memiliki aktivitas sebagai penurun tegangan permukaan. Biosurfaktan berasal dari mikroorganisme sehingga mempunyai sifat fisika kimia yang stabil, tidak mencemari lingkungan, sangat mudah terurai, dapat stabil pada temperatur tinggi, kadar asam dan garam tinggi, bahan baku dapat diperbaharui dan memiliki toksisitas rendah. Tujuan penelitian ini yaitu untuk menentukan letak gen berbagai jenis sampel biosurfaktan dari bakteri yang berpotensi memiliki aktivitas biosurfaktan. Pada penelitian ini dilakukan analisis penyejajaran antara gen bakteri Bacillus cereus dan Brevundimonas terrae dengan gen dari berbagai jenis senyawa biosurfaktan menggunakan metode bioinformatika dengan program BLAST. Dicari terlebih dahulu gen dari bakteri Bacillus cereus, Brevundimonas terrae, dan berbagai sampel biosurfaktan. Kemudian dilakukan analisis penyejajaran dua gen antara bakteri Bacillus cereus dan Brevundimonas terrae dengan berbagai sampel biosurfaktan. Hasil yang diperoleh yaitu berupa letak posisi genetik, persentase max score, query cover, e-value dan percent identity. Parameter yang menunjukkan tingkat homologi yaitu semakin tinggi query cover dan percent identity maka semakin tinggi tingkat homologinya. Hasil analisis bakteri Bacillus cereus dengan sampel biosurfaktan didapatkan Phospate acetyltransferase berada antara lokus 510000 hingga 580000, sampel ini memiliki tingkat homologi tertinggi dengan nilai query cover sebesar 100 % dan percent identitiy sebesar 98,46 %. Hasil analisis bakteri Brevundimonas terrae dengan sampel biosurfaktan didapatkan Lipoprotein berada antara lokus 280 hingga 612, sampel ini memiliki tingkat homologi dengan nilai query cover sebesar 28 % dan percent identitiy sebesar 77,81 %. Lipoprotein juga merupakan salah satu sampel biosurfakt. --- Biosurfactant is an alternative synthesis of surfactants that are synthesized extracellularly by microorganisms that have surface tension-lowering activity. Biosurfactants come from microorganisms that have stable physical and chemical properties, do not pollute the environment, are highly biodegradable, can be stable at high temperatures, high acid and salt levels, raw materials can measure and have low toxicity. The purpose of this study was to determine the location of the genes for various types of biosurfactants from bacteria that do not have biosurfactant activity. In this study, an alignment analysis was carried out between the genes of Bacillus cereus and Brevundimonas terrae with genes from various types of biosurfactant compounds using the bioinformatics method with the BLAST program. We searched first for genes from the bacteria Bacillus cereus, Brevundimonas terrae, and various samples of biosurfactants. Then analysis of the alignment of the two genes between the bacteria Bacillus cereus and Brevundimonas terrae with various samples of biosurfactants. The results obtained are in the form of the location, the proportion of the maximum score, the cover of the query, the e-value and the percent identity. Parameters that indicate the level of homology are the higher the cover query and the percent identity, the higher the homology level. The results of the analysis of Bacillus cereus bacteria with biosurfactant samples showed that phosphate acetyltransferase was between the locus 510000 to 580000, this sample had the highest homology level with a cover query value of 100% and an identity percentage of 98.46%. The results of the analysis of the Brevundimonas terrae bacteria with biosurfactant samples showed that Lipoproteins were between 280 to 612 loci, this sample had a homology level with a query cover value of 28% and an identity percentage of 77.81%. Lipoprotein is also one of the biosurfactant samples contained in the bacteria Bacillus cereus and Brevundimonas terrae.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Biosurfaktan, analisis penyejajaran, BLAST, Bacillus cereus, Brevundimonas terrae --- Biosurfactant, alignment analysis, BLAST, Bacillus cereus, Brevundimonas terrae
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions: Program Studi S1 Farmasi
Depositing User: pustakawan - -
Date Deposited: 03 Oct 2024 04:46
Last Modified: 03 Oct 2024 04:46
URI: http://repository.stfi.ac.id/id/eprint/1317

Actions (login required)

View Item View Item
Repository STFI is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.