VALIDASI METODE ANALISIS ISOLAT ANDROGRAFOLID DALAM SEDIAAN SUSPENSI MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS

AGISTY SALSABILA, - (2024) VALIDASI METODE ANALISIS ISOLAT ANDROGRAFOLID DALAM SEDIAAN SUSPENSI MENGGUNAKAN METODE SPEKTROFOTOMETRI UV-VIS. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Validasi metode analisis dilakukan untuk memastikan bahwa metode analitik akurat, spesifik, reprodusibel, dan toleran terhadap kisaran analit yang akan dianalisis. Penelitian ini bertujuan untuk menetapkan validasi metode analisis kadar andrografolid dalam sediaan suspensi menggunakan metode spektrofotometri UvVis dengan pelarut metanol : air (1:2) dan panjang gelombang 225 nm. Parameter validasi metode analisis yang ditentukan pada penelitian ini yaitu linearitas dan rentang, akurasi, presisi, batas deteksi dan batas kuantitasi. Hasil penelitian menunjukan koefisien korelasi yaitu 0,9995 dengan rentang konsentrasi 5 ppm – 80 ppm, batas deteksi 4,08 ppm, batas kuantitasi 12,38 ppm, akurasi 90,4%, dan presisi 0,003%. Hasil penetapan kadar andrografolid dalam sediaan suspensi yaitu sebesar 68,575% dengan nilai %RSD 0,006%. Berdasarkan hasil penelitian, tidak semua parameter validasi memenuhi persyaratan yang ditetapkan sehingga metode spektrofotometri Uv-Vis pada penelitian ini perlu dikembangkan kembali agar dapat diaplikasikan untuk penetapan kadar andrografolid dalam sediaan suspensi. ----- The validation of the analytical method was carried out to ensure that the method is accurate, specific, reproducible, and tolerant of the range of analytes to be analyzed. This study aims to establish the validation of the analytical method for determining andrographolide levels in suspension preparations using the UV-Vis spectrophotometry method with methanol : water (1:2) as the solvent and a wavelength of 225 nm. The validation parameters determined in this study include linearity and range, accuracy, precision, detection limit and quantitation limit. The results showed a correlation coefficient of 0.9995 with a concentration range of 5 ppm – 80 ppm, a detection limit of 4.08 ppm, a quantitation limit of 12.38 ppm, accuracy of 90.4%, and precision of 0.003%. The results of determining the levels of andrographolide in suspension preparations were 68.575% with a %RSD value of 0.006%. Based on the results, not all validation parameters met the established requirements, indicating that the UV-Vis spectrophotometry method in this study needs further development to be applicable for determining andrographolide levels in suspension preparations.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Andrografolid, spektrofotometri Uv-Vis, validasi metode analisis. ------ Andrographolide, UV-Vis spectrophotometry, analytical method validation
Subjects:	Q Science > QD Chemistry R Medicine > R Medicine (General)
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	28 Aug 2024 06:08
Last Modified:	28 Aug 2024 06:08
URI:	http://repository.stfi.ac.id/id/eprint/1098

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