RISMA FATIMAH APRILIANI, - (2024) DERIVATISASI KUERSETIN DENGAN DIMETIL SULFAT MELALUI REAKSI ETERIFIKASI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kuersetin adalah senyawa polifenol yang termasuk dalam kelompok flavonoid, Kuersetin dikenal memiliki berbagai aktivitas biologis yaitu dapat berfungsi sebagai antioksidan, antivirus, dan antiinflamasi. Penelitian ini dilakukan untuk menghasilkan senyawa turunan kuersetin dengan reaksi eterifikasi menggunakan dimetil sulfat. Proses derivatisasi dilakukan dengan mereaksikan kuersetin dan dimetil sulfat dalam kondisi basa menggunakan NaOH 5N. Hasil reaksi kuersetin dipisahkan menggunakan Ekstraksi Cair-Cair (ECC) dan dimurnikan menggunakan kromatografi kolom. Derivat kuersetin diidentifikasi menggunakan Kromatografi Lapis Tipis (KLT), Spektrofotometri UV-Visibel, Fourier Transform Infrared (FTIR) serta diuji aktivitas antioksidannya menggunakan metode DPPH. Hasil penelitian menunjukkan persentase rendemen produk derivat sebesar 59,59%. Hasil dari KLT menunjukkan adanya perubahan noda dan nilai Rf antara isolat kuersetin dengan hasil derivat. Hasil spektrum UV-Visibel menunjukkan adanya pergeseran panjang gelombang maksimum pada puncak serapan pertama dari 256 nm menjadi 251 nm dan pada puncak serapan kedua dari 352 nm menjadi 334 nm. Hasil spektrum FTIR menunjukkan adanya gugus baru dan perbedaan pada bilangan gelombang yang dihasilkan. Pada uji aktivitas antioksidan, derivat kuersetin memiliki aktivitas antioksidan yang lemah dengan nilai IC50 sebesar 136 µg/mL dibandingkan kuersetin dengan nilai IC50 sebesar 6 µg/mL. Derivat kuersetin dapat dibentuk melalui reaksi eterifikasi. ----- Quercetin is a polyphenolic compound which is included in the flavonoid group. Quercetin is known to have various biological activities, namely it can function as an antioxidant, antiviral and anti-inflammatory. This research was carried out to produce quercetin derivative compounds by etherification reaction using dimethyl sulfate. The derivatization process was carried out by reacting quercetin and dimethyl sulfate under alkaline conditions using 5N NaOH. The quercetin reaction product was separated using Liquid-Liquid Extraction (ECC) and purified using column chromatography. Quercetin derivatives were identified using Thin Layer Chromatography (TLC), UV-Visible Spectrophotometry, Fourier Transform Infrared (FTIR) and tested for antioxidant activity using the DPPH method. The research results showed that the yield percentage of derivative products was 59.59%. The results of TLC showed changes in staining and Rf values between the quercetin isolate and the derivative results. The UVVisible spectrum results show a shift in the maximum wavelength at the first absorption peak from 256 nm to 251 nm and at the second absorption peak from 352 nm to 334 nm. The FTIR spectrum results show the presence of new clusters and differences in the resulting wave numbers. In the antioxidant activity test, the quercetin derivative had weak antioxidant activity with an IC50 value of 136 µg/mL compared to quercetin with an IC50 value of 6 µg/mL. Quercetin derivatives can be formed through etherification reactions.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | kuersetin, dimetil Sulfat, eterifikasi, derivatisasi. ---- quercetin, dimethyl Sulfate, etherification, derivatization |
| Subjects: | Q Science > QD Chemistry R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 28 Aug 2024 04:29 |
| Last Modified: | 28 Aug 2024 04:29 |
| URI: | http://repository.stfi.ac.id/id/eprint/1095 |
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