PENAPISAN BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI DADIH SUSU SAPI MURNI

ONE MARSWARI OKSU, - (2024) PENAPISAN BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI DADIH SUSU SAPI MURNI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Bakteri Asam Laktat (BAL) memiliki peran penting dalam sistem pencernaan manusia, terutama dalam menghambat pertumbuhan bakteri patogen dan menjaga keseimbangan mikroflora usus. Beberapa BAL juga menghasilkan biosurfaktan, surfaktan alami yang diproduksi oleh mikroorganisme indigen. Penelitian ini bertujuan untuk mengisolasi BAL penghasil biosurfaktan yang diisolasi dari dadih susu sapi murni. Isolasi BAL dilakukan melalui teknik pengenceran bertingkat menggunakan larutan NaCl 0,9%, dengan sampel diencerkan hingga 10-6 dan ditumbuhkan pada media MRS Agar. Karakterisasi isolat BAL mengikuti Bergey's Manual of Determinative Bacteriology, meliputi karakteristik morfologi dan biokimia. Pada isolate bakteri selanjutnya, dilakukan penapisan uji biosurfaktan, diantaranya uji emulsifikasi, oil spreading, dan drop collapse. Hasil penelitian menunjukkan bahwa dari 4 isolat BAL yang diperoleh, semuanya bersifat Gram positif. Uji katalase menghasilkan 1 isolat positif (isolat 4) dan 3 isolat negatif (51, 52, dan 6). Karakterisasi morfologi menunjukkan 2 isolat berbentuk bulat dan 2 isolat berbentuk batang. Karakterisasi fisiologi dan biokimia menunjukkan bahwa semua isolat memiliki karakteristik katalase negatif, bersifat homofermentatif dan heterofermentatif, termofilik dan mesofilik, serta mampu tumbuh pada konsentrasi NaCl 4% - 6,5%. Berdasarkan hasil karakterisasi, dapat disimpulkan bahwa dari keempat isolat bakteri asam laktat yang dapat menghasilkan biosurfaktan adalah isolat 51, 52, dan 6 dengan karakteristik morfologi, fisiologi dan biokimia, sehingga diduga 3 isolat berasal dari genus Lactobacillus spp. (51, 52, dan 6) dan 1 isolat genus Streptococcus spp. ---- Lactic Acid Bacteria (BAL) have an important role in the human digestive system, especially in inhibiting the growth of pathogenic bacteria and maintaining the balance of the intestinal microflora. Some BALs also produce biosurfactants, naturally occurring surfactants produced by indigen microorganisms. This study aims to isolate biosurfactant-producing BAL isolated from pure cow's milk curd. Isolation of BAL was carried out through a cascade dilution technique using a 0.9% NaCl solution, with the sample diluted to 10-6 and grown on MRS agar medium. The characterization of BAL isolates follows Bergey's Manual of Determinative Bacteriology, covering morphological and biochemical characteristics. In the next bacterial isolate, biosurfactant tests are filtered, including emulsification tests, oil spreading, and drop collapse tests. The results showed that of the 4 BAL isolates obtained, all of them were Gram-positive. The catalase test produced 1 positive isolate (isolate 4) and 3 negative isolates (51, 52, and 6). Morphological characterization showed 2 spherical isolates and 2 rod-shaped isolates. Physiological and biochemical characterizations show that all isolates have negative catalase characteristics, are homofermentative and heterofermentative, thermophilic and mesophilic, and are able to grow at NaCl concentrations of 4% - 6.5%. Based on the results of characterization, it can be concluded that of the four isolates of lactic acid bacteria that can produce biosurfactants, they are isolates 51, 52, and 6 with morphological, physiological and biochemical characteristics, so it is suspected that 3 isolates come from the genus Lactobacillus spp. (51, 52, and 6) and 1 isolate of the genus Streptococcus spp.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Dadih, Bakteri Asam Laktat (BAL), isolasi, Biosurfakan. ----- curds, Lactic Acid Bacteria (BAL), insulation, biosurfactants
Subjects:	Q Science > Q Science (General)
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	28 Aug 2024 01:36
Last Modified:	28 Aug 2024 01:36
URI:	http://repository.stfi.ac.id/id/eprint/1072

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