ALIFIA TIARA DETFI, - (2024) ANALISIS KEMOMETRIK PROFIL SPEKTRUM FOURIER TRANSFORM INFRARED TANAMAN SAMBILOTO (Andrographis paniculata Nees.) DARI BEBERAPA DAERAH DI JAWA BARAT. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Sambiloto (Andrographis paniculata Nees.) dikenal sebagai tumbuhan obat tradisional dengan khasiat farmakologis signifikan, termasuk antiinflamasi, antiviral, dan antimikroba, berkat kandungan andrografolid. Tujuan dari penelitian ini adalah untuk memperoleh data ilmiah mengenai kandungan metabolit sekunder, kualitas simplisia, serta profil spektrum FTIR tanaman sambiloto dari berbagai daerah di Jawa Barat dengan pengelompokkannya berdasarkan PCA dan analisis kluster. Berdasarkan pengujian standarisasi simplisia, sambiloto memiliki rentang kadar sari larut air 11,56%-23,60%, kadar sari larut etanol 5,51%-8,77%, kadar abu total 5,61%-10,49%, susut pengeringan 0,27%-0,91%, dan kadar air 2,60%-4,15%. Secara kimia, tanaman sambiloto dari beberapa daerah tersebut mengandung alkaloid, flavonoid, tanin, fenol, saponin, kuinon, steroid, dan triterpenoid. Analisis spektrum IR pada rentang 4000–400 cm-1 menghasilkan 8 puncak khas yang mencakup gugus O-H (alkohol), C-H (alkana), C=O (keton), C=C (alkena), -CH3 (alifatik), C-O (alkohol), dan C=H (alkena). Hasil PCA menunjukkan plot score untuk sampel daun dengan PC-1 (99%) dan PC-2 (0%), batang dengan PC-1 (87%) dan PC-2 (5%), serta kombinasi daun dan batang dengan PC-1 (97%) dan PC-2 (2%). Pengelompokan PCA menghasilkan 4 kelompok pada sampel daun: Subang Arcamanik, Bogor-Pagarsih-Depok, Kopo-Parompong, dan Sumedang. Untuk sampel batang, terdapat 4 kelompok: Subang-Parompong, Pagarsih-BogorArcamanik, Depok-Kopo, dan Sumedang. Analisis PCA pada kombinasi daun dan batang menunjukkan bahwa PCA dapat membedakan sampel berdasarkan jenisnya. Analisis kluster menunjukkan bahwa sampel daun dari Bogor dan Pagarsih, batang dari Kopo dan Depok, serta kombinasi daun-batang dari Kopo dan Sumedang memiliki jarak terdekat, menunjukkan bahwa sampel memiliki kesamaan karakteristik yang tinggi. Hasil penelitian ini menunjukkan bahwa metode analisis kemometrik profil spektrum FTIR dapat digunakan untuk mengelompokkan profil spektrum FTIR dari berbagai daerah di Jawa Barat. ---- Sambiloto (Andrographis paniculata Nees.) is recognized as a traditional medicinal plant with notable pharmacological properties, including anti-inflammatory, antiviral, and antimicrobial effects, largely due to its andrographolide content. This study aimed to gather scientific data on the secondary metabolite content, simplicia quality, and FTIR spectrum profile of sambiloto from various regions in West Java, and to classify them using PCA and cluster analysis. In the simplicia standardization, sambiloto exhibited water-soluble extract content ranging from 11.56% to 23.60%, ethanol-soluble extract content from 5.51% to 8.77%, total ash content from 5.61% to 10.49%, drying loss from 0.27% to 0.91%, and moisture content from 2.60% to 4.15%. Chemically, sambiloto from several regions contained alkaloids, flavonoids, tannins, phenols, saponins, quinones, steroids, and triterpenoids. IR spectrum analysis in the range of 4000–400 cm⁻¹ revealed eight characteristic peaks, including O-H (alcohol), C-H (alkane), C=O (ketone), C=C (alkene), -CH₃ (aliphatic), C-O (alcohol), and C=H (alkene) groups. PCA results indicated score plots for leaf samples with PC-1 (99%) and PC-2 (0%), stem samples with PC-1 (87%) and PC-2 (5%), and combined leaf and stem samples with PC-1 (97%) and PC-2 (2%). PCA grouping resulted in four groups for leaf samples: Subang-Arcamanik, Bogor-Pagarsih-Depok, Kopo-Parompong, and Sumedang. For stem samples, there were four groups: Subang-Parompong, Pagarsih-Bogor-Arcamanik, Depok-Kopo, and Sumedang. PCA analysis of the combined leaf and stem samples indicated that PCA could distinguish the samples based on their types. Cluster analysis showed that leaf samples from Bogor and Pagarsih, stem samples from Kopo and Depok, and combined leaf-stem samples from Kopo and Sumedang had the closest similarities, indicating high similarity characteristics. This study demonstrated that the chemometric analysis of FTIR spectrum profiles could effectively classify samples from various regions in West Java.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Tanaman Sambiloto (Andrographis paniculata Nees.), FTIR, Kemometrik, PCA, Analisis Kluster. ----- Sambiloto (Andrographis paniculata Nees.), FTIR, Chemometrics, PCA, Cluster Analysis. |
| Subjects: | Q Science > QD Chemistry R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 26 Aug 2024 04:17 |
| Last Modified: | 26 Aug 2024 04:17 |
| URI: | http://repository.stfi.ac.id/id/eprint/1056 |
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